easily degradating and various isomeric forms of rapamycin (sirolimus) face the determination of this compound to many challenges. in this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (rp-hplc) method for rapamycin. separation was performed on a c8 column (mz, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

a gradient reversed-phase high performance liquid chromatography (hplc) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (gnrh) antagonistic activity used in infertility treatment. the hplc method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ich guidelines. the chromatograph...

the present study was designed to develop a simple, validated liquid chromatographic method for the analysis of azithromycin in bulk and pharmaceutical dosage forms using ultraviolet detector. the best stationary phase was determined as c18 column, 5 µm, 250 mm × 4.6 mm. mobile phase was optimized to obtain a fast and selective separation of the drug. flow rate was 1.5 ml/min, wavelength was se...

a sensitive procedure for determining total vitamin c (ascorbic acid + dehydrate ascorbic acid) in a blood drop from a finger prick, before and after the adminis- tration of a vitamin c tablet is described. analysis was carried out by high per- formance liquid  chromatography with  electrochemical detection (hplc/ec). measurements were taken one hour, two hours and six hours after the administr...

an environmentally benign rp-hplc approach for rapid analysis of glibenclamide in pure form, developed nanoemulsion and commercial tablets was developed and validated in present investigation. the green chromatographic identification was performed on lichrosphere 250 x 4.0 mm rp c8 column having a 5µm packing as a stationary phase using a combination of ethanol: methanol (50:50 % v/v) as a mobi...

the pharmacokinetic parameters of domestic and imported ranitidine hydrochloride tablets (ranitidine, formulated and manufactured by kharazmi pharmaceuticals, iran, and zantac ? manufactured by glaxo, uk) were measured in 14 healthy subjects following oral administration of a single 300-mg dose of each brand and compared for bioequivalence evaluation. the pharmaceutical equivalency of both form...

a simple and reproducible hplc method with spectrophotometric detection was developed for determination of rivastigmine in human plasma. liquid-liquid extraction of rivastigmine and donepezil (as internal standard) from plasma samples was performed with 1-butanol/n-hexane (2:98 v/v) in alkaline condition followed by back-extraction into diluted acetic acid. chromatography was carried out using ...

Absorption spectrophotometry has been and still is the industry standard for detection in HPLC. Limit of detection (LOD) and linear dynamic range (LDR) are the primary performance requirements and have driven continuous improvement of spectrophotometric HPLC detectors. Recent advances in HPLC column technology have led to low flow-rate HPLC such as capillary HPLC and nanoflow HPLC and put highe...

The use of luminescence techniques for detection in HPLC has high potential. These techniques are especially useful when extremely high sensitivity and/or selectivity are required, and they can be nicely adapted to the demands of miniaturised HPLC. Three aspects are discussed in the present paper: (i) on-line post-column derivatisation in HPLC with conventional fluorescence detection; (ii) the ...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...